Fluorescence Diagram. The jablonski diagram typically used to illustrate fluorescence in molecular spectroscopy demonstrates the excited states of a molecule along with the radiative and non radiative transitions that can occur between them. 1 shows the jablonski diagram jablonski 1933 a schematic of the transition of electronic state of a molecule during.
Jablonski diagram illustrating the processes involved in the creation of an excited electronic singlet state by optical absorption and subsequent emission of fluorescence. A plot of emission against wavelength for any given excitation. The energy levels can be quantitatively denoted but most of these diagrams use energy levels schematically.
The energy levels can be quantitatively denoted but most of these diagrams use energy levels schematically.
1 shows the jablonski diagram jablonski 1933 a schematic of the transition of electronic state of a molecule during. Electrons are normally at the lowest energy state indicated by s 0. The vertical illuminator in the center of the diagram has the light source at one end episcopic lamphouse and the filter cube at the other. Fluorescence emission by the specimen primarily red wavelengths which results from the green light excitation is gathered by the objective and passes through the dichromatic mirror and barrier filter light above cut off in figure 2 c.