Epifluorescence Microscope Diagram. Epifluorescence microscopy the majority of fluorescence microscopes especially those used in the life sciences are of the epifluorescence design shown in the diagram. The confocal image required 2 hours to build in a point scanning system and the widefield image was collected with an integration.
The fluorescence microscope figure 3 is a schematic diagram of a typical epifluorescence microscope which uses incident light ie episcopic illumination. In this figure the optical path of an epifluorescence microscope. Two micro lens arrays in the illumination path of a widefield epifluorescence microscope can produce an illumination coefficient of variation between 1 and 2 with high uniformity.
In this figure the optical path of an epifluorescence microscope.
The goal of the fluorescence microscope is to separate emitted light dim from excitation light bright fig. Light of the excitation wavelength is focused on the specimen through the objective lens. Two mla were inserted between the relay lens and the filter cube gray. The confocal image required 2 hours to build in a point scanning system and the widefield image was collected with an integration.